50.    Argentinian multicenter study on urinary tract infections due to Streptococcus agalactiae in adult patients

Background: S. agalactiae (GBS) is a recognized urinary pathogen of both pregnant or non-pregnant women and men. Unfortunately actual reports of serotype distribution and antimicrobial susceptibility rates of Argentina are limited. Methods: Between 1/07/14 and 30/06/15, a multicenter study with 87 centers was performed in Argentina to assess the susceptibility to penicillin and fluoroquinolones of GBS isolated from the urinary tract of adult patients. Seven hundred and one isolates were sent to the reference laboratory in such period, but only 211 fulfilled our selection criteria (availability of demographic data, underlying diseases reported, colony counts greater than 10 5 CFU/ml, unique organism isolated from the urine sample). Patients have been stratified in males (M) (n = 28), non-pregnant women (NPW) (n = 104) and PW (n = 79) and clinical data was analysed as well. One hundred and ninety four viable isolates were tested by the disc diffusion method with disks of ceftibuten, oxacillin, ceftizoxime (Kimura scheme for penicillin resistance screening), levofloxacin and norfloxacin. Serotyping was performed by using the Strep B latex kit (Statens Serum Institut, Denmark). Results: No penicillin
resistant GBS was found, but 25/194 (12.8%) were resistant to both fluoroquinolones. One hundred eighty four isolates could be serotyped. Serotype Ia was prevalent among GBS isolated in UTI of adult patients (40.2%). Serotype III was detected more frequently among PW than in NPW (p < 0.05). Globally, serotypes Ia, Ib, and III, represented the 83.2% of isolates. Diabetes mellitus was the most frequent comorbility associated with UTI due to GBS. Conclusion: Fluoroquinolone resistance is increasing in Argentina, and it is very important to highlight the high percentage observed in non-pregnant women since they are frequently used for the empiric treatment of urinary tract infections in such population.